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Please take this quick survey to tell us about what happens after you publish a paper. The proteins were blotted onto cellulose nitrate sheets and BF was detected by incubation of these sheets with anti-BF serum, anti-IgG serum, and I-labeled protein A successively. After autoradiography, four or five main BF bands were found in plasma of male mice. In backcross and F 2 generations the BF variants always cosegregated with the H-2 haplotypes. In this way linkage to H-2 could be established. When the electrophoretic BF patterns of males and females were compared, a sexual dimorphism was discovered; the females of each strain had only three main BF bands compared with the four or five found in males.
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